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KMID : 0624620190520060391
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BMB Reports
2019 Volume.52 No. 6 p.391 ~ p.396
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CCAAT/enhancer-binding protein beta (C/EBPβ) is an important mediator of 1,25 dihydroxyvitamin D3 (1,25D3)-induced receptor activator of nuclear factor kappa-B ligand (RANKL) expression in osteoblasts
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Jo Sung-Sin
Lee Yun-Young
Han Jin-Il
Lee Young-Lim
Yoon Su-Bin
Lee Jae-Hyun
Oh Youn-Seo
Han Joong-Soo
Sung Il-Hoon
Park Ye-Soo
Kim Tae-Hwan
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Abstract
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Receptor activator of nuclear factor kappa B ligand (RANKL) expression in osteoblasts is regulated by 1,25-dihydroxyvitamin D3 (1,25D3). CCAAT/enhancer-binding protein beta (C/EBPβ) has been proposed to function as a transcription factor and upregulate RANKL expression, but it is still uncertain how C/EBP? is involved in 1,25D3-induced RANKL expression of osteoblasts. 1,25D3 stimulation increased the expression of RANKL and C/EPBβ genes in osteoblasts and enhanced phosphorylation and stability of these proteins. Moreover, induction of RANKL expression by 1,25D3 in osteoblasts was downregulated upon knockdown of C/EBPβ. In contrast, C/EBPβ overexpression directly upregulated RANKL promoter activity and exhibited a synergistic effect on 1,25D3-induced RANKL expression. In particular, 1,25D3 treatment of osteoblasts increased C/EBPβ protein binding to the RANKL promoter. In conclusion, C/EBPβ is required for induction of RANKL by 1,25D3.
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KEYWORD
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1,25-dihydroxyvitamin D3 (1,25D3), C/EBPβ, Osteoblasts, RANKL
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